Acridine Orange Stain Dye - 236ml (8fl.oz) Microscopy Stain

US $200

Swadlincote, Derbyshire, United Kingdom
Jun 4th

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Acridine Orange Stain 236ml (8fl.oz) Acridine is used in many different areas, such as epifluorescence microscopy and the assessment of sperm chromatin quality. Acridine orange stain is particularly useful in the rapid screening of normally sterile specimens, and its recommended for the use of fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. The staining has to be performed at an acid pH in order to obtain this differential staining effect with bacteria showing orange stain and tissue components yellow to green. Acridine orange is a versatile fluorescence dye to stain acidic vacuoles (lysosomes, endosomes, and autophagosomes), RNA, DNA in living cells. This method is a cheap and easy way to study lysosomal vacuolation, autophagy, and apoptosis. Acridine orange emits from yellow, orange, to red fluorescence when the pH get lower in the acidic vacuole of the living cell. Acridine orange emits yellow fluorescence when it binds RNA and green fluorescence when it binds DNA. Nucleus emit yellowish-green fluorescence in normal condition, and deep green fluorescence when the RNA synthesis is inhibited by inhibitors such as chloroquine. Acridine orange can be used in conjunction with ethidium bromide to differentiate between viable, apoptotic and necrotic cells. Additionally, Acridine orange may be used on blood samples to cause bacterial DNA to fluoresce, aiding in clinical diagnosis of bacterial infection once serum and debris have been filtered. Quality Guaranteed. Our stains are professional diagnostic quality and are used in NHS hospital laboratories, research institutions and Universities in the UK. For more stains, bulk reagents and diagnostic products, please contact us.