Oxford 450 Dilutor, Diluter 1000 Ml, 20ul Preset Sample, 1-10ml (set By User)

US $124.20

  • Palm Desert, California, United States
  • Jan 30th
Brand new Oxford 450 Dilutor, Each. Product No. 8885-045008 List Price $500 (Additional units can be purchased at HunterScientificSupplies.com) PRINCIPLE OF OPERATION The Oxford 450 Dilutor is a general purpose, bottle mounted instrument that will aspirate a 20ul sample into glass pipette tip, then deliver this sample together with an adjustable amount of diluent from the reservoir bottle into receiving vessel.  Lubricant and detailed operating instructions are included. SPECIFICATIONS The precision is expressed as a coefficient of variation at one standard deviation and is verified gravimetrically using deionized water at 22 deg. +- 3 deg. Celsius. Precision 1% CV Accuracy - user set The Oxford 450 Dilutor has the following features: 1. Sample size - preset at 20uL; 2.  Diluent volume set by user, with range of 1 to 10 ml; 3.  Only glass, Teflon, ceramic and polypropylene come in direct contact with diluent and/or sample; 4.  Sensitive parts are internally mounted to reduce breakage; and 5.  Modular construction permits repair and replacement of any part without the necessity of factory return. SET UP AND OPERATING INSTRUCTIONS (Refer to Fig. 1) Carefully remove the unit from its carton and examine for breakage.  Any defects should be reported immediately to your dealer. Install the glass pipette tip as follows.  With one hand, gently grip the pipette tip near the non-tapered end and, with the other hand, brace the upper plastic connector.  Using a twisting motion, insert the tip approximately 3/8" into the vacant outlet port of the upper plastic connector.  Fill the reservoir with desired diluent to within 1 3/4" of the top.  Maximum capacity is approximately 1000 ml.  Insert the assembly into the reservoir and hand tighten the lid.  Loosen the screw on the indicator stop and set to the desired diluent volume by aligning the top edge of the indicator stop with the appropriate graduation line on the diluent scare post.  Tighten the screw. CAUTION: Always remove the tip cap before operating the diluent plunger. To prime, pump the diluent plunger several slow strokes.  Then lean the complete unit about 25 degrees toward the plunger and make several more slow strokes in this position to remove all air from the syringe barrel.  Return the unit to an upright position and continue pumping until the pipette tip is fully charged.  A 5 mm vent is provided for the attachment of filtering and drying devices, if so desired. ALWAYS WIPE THE PIPETTE TIP CLEAN BEFORE INTRODUCING IT INTO A SAMPLE TO PREVENT CONTAMINATING THE SAMPLE. Immerse the pipette tip 1/8 to 1/4 inch into the sample and smoothly rotate the sample knob from the primary stop to the secondary stop.  Note that as you move the sample knob a red warning inert is revealed.  IT IS ESSENTIAL THAT THE SAMPLE INTAKE BEGIN WITH THE KNOB FIRMLY AGAINST THE PRIMARY STOP.  NONE OF THE RED INSERT SHOULD SHOW. Withdraw the sample container and carefully wipe the pipette tip.  Do not touch the end of the tip.  Place the desired receiving vessel under the tip and smoothly lift the diluent plunger until it meets the indicator stop.  Pause slightly.  Remove any droplets from the tip.  Then gently push the diluent plunger down until it bottoms firmly and tip off any droplet on the tip into the receiving vessel.  This will deliver the sample and the diluent and perform a flushing action to remove all traces of the sample from the tip.  Return the sample knob to the primary stop position and wipe the pipette tip dry.  Maximum reproducibility is achieved when a slow, steady stroke and consistent technique are used.  Excessive speed may cause the unit to pre-dispense or post-dispense a droplet.  When dilutor is not in use, always recap the pipette tip.  If, for any reason, the diluent withdraws, reprime before using it. OPERATING PRECAUTIONS If a refrigerated reagent is used, equalize the temperature of the dilutor before filling by refrigerating the unit 2 to 4 hours.  Failure to do so may result in damage to the dilutor. When refilling the reservoir, keep the inner assembly upright to avoid the necessity of repriming. Make sure the tip cap is removed before dispensing.  This prevents spraying of the reagent if the tip cap is forced off by the syringe plunger action. When dispensing hazardous or viscous materials, DO NOT apply force to the plunger greater than that required to dispense water.  Breakage or separation of the discharge line could result in personal injury. Periodically check integrity of the reservoir bottle.  Glass bottles with surface imperfections could fail due to chemical attack (stress corrosion). Withdrawal of the liquid in the tip during upward movement of the diluent plunger can be caused by dirty ball valves and indicates that cleaning is needed.  Never allow the diluent to dry out in the instrument as the plunger may jam in the barrel.  During long periods of disuse, thoroughly clean and dry the dilutor.   (See Cleaning and Maintenance section.) CHEMICAL COMPATIBILITY The dilutor parts that come in direct contact with reagents have excellent compatibility except for alkalies, elemental fluorines, fluorine precursors, perchloric and hydrofluoric acids. CLEANING AND MAINTENANCE Occasionally remove, with a moistened swab, any residue which may collect at the top of the diluent plunger and barrel.  Reagents which tend to deposit heavy residue (such as NaOH) require more frequent attention.  If operation indicates that the ball valves are dirty, select a suitable solvent and flush the instrument by pumping the solvent through the complete unit.  Occasionally the use of sulfuric acid dichromate solutions or commercial glass cleaner may be required.  WARNING: When using sulfuric acid dichromate solution, use protective equipment for eyes and skin.  If the sulfuric acid dichromate solution contacts the eyes or skin, rinse with copious amounts of water. After the unit has been cleaned, flush with warm water and a mild detergent, followed by alcohol to speed the drying the process.  If necessary, apply sparingly a thin, even coat of silicone stopcock grease to only the upper 1/2" of the diluent plunger.  Use only the lubricant supplied with the unit.  Do not use a high vacuum type grease as it is too viscous. Do not apply grease to the barrel as it is difficult to remove any excess.  Excess grease may make the plunger stiff to operate.  If the sample knob does not rotate smoothly, remove the sample knob and apply a small amount of silicone grease to the threads of the sample plunger (see Figure 2).  If complete disassembly is desired for cleaning, refer to the disassembly procedure. If, for any reason, the diluent plunger is jammed in the barrel, follow the disassembly procedure until the scale post (3) and indicator stop (4) are removed.  Submerge the remaining assembly in hot water until the plunger can be freed with a slight twisting motion while gripping the barrel from below.  Use normal precautions to prevent injury should the glass break. DISASSEMBLY PROCEDURE. (Refer to Figure 2) Remove the complete assembly from the reservoir.  Rinse and flush as much diluent as possible from the glassware. Gently twist off the pipette tip (11), gripping it as near the upper plastic connector (10) as possible.  Loosen the set screw (13) and remove the sample knob (1) and sample knob spring (23). Then remove each of the following parts, the order given: Threaded sample plunger (2) (remove by unscrewing) Upper mounting assembly screw (14) Lower mounting assembly screw (19) Upper positioner (15) Threaded swivel ball (16) Scale post mounting screw (17) Lower positioner (18) Scale post (3), with indicator stop (4) attached Diluent plunger (5) NOTE: Plunger and barrel are a matched set and each should be marked to prevent mismatching whenever they are separated. Detached the sample syringe barrel (9) and the upper plastic connector (10) together by gently twisting while supporting the ball valve assembly (8) from below. Next grip the ball valve assembly (8) and diluent syringe barrel (7) at the lower Teflon connector (22).  Pull very gently to separate the parts.  Heating in very hot water will ease this separation.  If replacing the lower Teflon connector, it may be cut with a sharp knife or razor blade to ease removal. Heat the inner lid (6) in very hot water.  Carefully detach the ball valve assembly (8) and diluent syringe barrel (7) from their fittings. To assemble: Reverse the disassembly procedure.  When inserting the diluent barrel (7) into the inner lid (6), make certain the top of the barrel is even with the top of the upper surface of the inner lid.  The lower side port of the barrel must be in line with the side port of the ball valve assembly from below when installing the upper plastic connector and sample syringe barrel.  Do not force the diluent plunger into the barrel as it can be cracked. Oxford 450 Dilutor Assembly 1.  Sample Knob 2.  Threaded Sample Plunger 3.  Scale Post 4.  Indicator Stop and Thumb Screw 5.  Diluent Plunger and Handle 6.  Inner Lid 7.  Diluent Syringe Barrel 8.  Ball Valve Assembly 9.  Sample Syringe Barrel 10.  Upper Plastic Connector 11.  Pipette Tip 12.  Pipette Tip Cap 13.  Sample Knob Screw 14.  Upper Mounting Assembly Screw 15.  Upper Positioner 16.  Swivel Ball 17.  Scale Post Mounting Screw 18.  Lower Positioner 19.  Lower Mounting Assembly Screw 20.  Rear Support 21.  Outer Lid 22.  Lower Teflon Connector 23. Sample Knob Spring FREE! Sellers: Add a FREE map to your listings. FREE!
Condition:
New: A brand-new, unused, unopened, undamaged item in its original packaging (where packaging is applicable). Packaging should be the same as what is found in a retail store, unless the item is handmade or was packaged by the manufacturer in non-retail packaging, such as an unprinted box or plastic bag. See the seller's listing for full details. ...
Brand Oxford
MPN 8885-045008 8885045008
Model 450 Dilutor Diluter 1-10 ml User Set, 20ul Preset
Country/Region of Manufacture United States

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