Recombinant Human Il-21 10ug Expressed In Human Cells For T Cell Study

US $49.99

Livermore, California, United States
Jul 10th

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Summer Promotion Recombinant Human IL-21 Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin High Purity Animal Free and Xeno Free Tag Free Source: Human cells derived Structure: Glycosylated monomer Purity: >95% by SDS-PAGE Endotoxin Level: <0.1EU/ug Molecular Weight: ~15.4kDa Formulation: Lyophilized from a 0.2m filtered solution in PBS without carrier protein Activity Assay The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml. The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml. Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution. Protein Description IL-21 is a typical family I cytokine with broad pleiotropic actions and is primarily produced by T follicular helper cells,Th17, and natural killer T cells in response to antigenic stimulation. Il-21 generally enhances antigen-specific responses of immune cells. It controls the activation, proliferation, differentiation, cytotoxicity, and survival of various target immune cells. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues. References Parrish Novak, et al. (2000) Nature 408,57. Spolski R, et al.(2008) Annu Rev Immunol 26,57. Mehta DS, et al.(2004) Immune Rev 202,84.

Condition:	New: A brand-new, unused, unopened, undamaged item in its original packaging (where packaging is applicable). Packaging should be the same as what is found in a retail store, unless the item was packaged by the manufacturer in non-retail packaging, such as an unprinted box or plastic bag. See the seller's listing for full details. ...	Custom Bundle	No
Common Name	Interleukin 21
Volume/Quantity	10ug
Brand	StemImmune LLC
MPN	HCT-I21-0010
Intended Use/Discipline	Anesthesiology, Biological Laboratory, Cardiology, Cosmetology, Dental Laboratory, Dentistry, Dermatology, Emergency Medicine, Endocrinology, Endodontics
UPC	4913263157403